Service facilities

Translational Research Initiatives in Pathology (TRIP) Laboratory

Location

1111 Highland Ave
53792 Madison , WI
Phone: 608-265-1093
Wisconsin
Translational Research Initiatives in Pathology
Location Information
Street Address: 
Wisconsin Institute for Medical Research
1111 Highland Ave
53792 Madison , WI
Phone: 608-265-1093
US
Facilities: 
 
The Translational Research Initiatives in Pathology (TRIP) Lab provides support to pathology faculty and trainees working on translational and collaborative research projects; and partners with the UW Carbone Cancer Center’s Biobank as part of their Translational Science BioCore (TSB). In addition, the laboratory offers high-quality histological, molecular/morphometric analyses and consultative services such as tissue arrays, laser dissection microscopy, quantitative morphometric analyses via imaging, and IHC/ISH on a fee for service basis available to the campus community and beyond.

Mission:

The mission of the TRIP Lab is to provide quality services in cellular, molecular and quantitative pathology for members of the Department of Pathology, the University of Wisconsin Carbone Cancer Center, the Medical Center and the University of Wisconsin- Madison campus.

The specific goals of the TRIP Lab include:

 

  1. Facilitate translational research focusing on the pathologic basis of human diseases.
  2. Provide state of the art services for users of the laboratory.
  3. Become the regional center of excellence for molecular testing in human diseases and in quantitative pathology

Service Request Form

Policies
Contact Name: 
Sally Ann Drew, BS, MT
Phone number: 
608-265-1093

UW-Madison Optical Imaging Core (UWOIC) Facility

Location

1111 Highland Avenue
53705 Madison , WI
Wisconsin
UW-Madison Optical Imaging Core (UWOIC) Facility
Location Information
Street Address: 
Rooms 2071-2076 WIMR2
1111 Highland Avenue
53705 Madison , WI
US
Facilities: 

Currently, the core offers imaging services on three microscopes systems, (1) a Leica SP8 3X STED Super-resolution microscope, (2) Nikon A1RS confocal microscope, and (3) Leica DMi8 inverted fluorescent microscope. 


Imaging techniques available through the core now include:  Standard fluorescence, Super resolution microscopy, live cell long-term imaging, deconvolution, particle tracking, fluorescence spectral detection, FRET , FRAP, photactivation/uncaging, and calcium imaging.  The Optical Core also includes a small library of labeled secondary antibodies, miscellaneous reagents and mounting mediums.  Investigators can maintain and grow live cells in a culture room equipped with biosafety cabinets and CO2 incubators.

Usage fee for the Leica STED - $30/hr
Usage fee for the Nikon A1 - $30/hr
Usage fee for the Leica widefield microscope - $10/hr

Lance Rodenkirch 
L5/177 CSC WIMR
608-263-3248

Contact Name: 
Lance Rodenkirch
Phone number: 
608-263-3248

UWCCC Experimental Pathology Laboratory

Location

1111 Highland Ave.
53705 Madison , WI
Phone: 608-262-1836
Wisconsin
UWCCC Experimental Pathology Laboratory
Location Information
Street Address: 
WIMR I Rooms 4012, 4016-4018
1111 Highland Ave.
53705 Madison , WI
Phone: 608-262-1836
US
Facilities: 

The Experimental Pathology Laboratory (EPL) provides histology services for UW Carbone Cancer Center (UWCCC) members, non-members and non-UW researchers.

Histology services

  • Preparation of histologic sections from both paraffin-embedded and frozen tissue, including serial sections
  • Block preparations of cell pellets and embryoid bodies
  • Wide variety of routine and specialized histotechnology methods
  • H&E
  • Enzyme histochemistry
  • Immunohistochemistry
  • Special stains
  • Slide preparation for in situ hybridization, LCM, and audioradiography
  • Pathology consultation

 

 

Policies
Contact Name: 
Ruth Sullivan
Joe Hardin
Ella Ward
Phone number: 
608-262-1836

UWCCC Flow Cytometry Laboratory

Location

1111 Highland Ave
53705 Madison , WI
Phone: 608-263-0313
Wisconsin
UWCCC Flow Cytometry Laboratory
Location Information
Street Address: 
7016 Wisconsin Institutes for Medical Research
1111 Highland Ave
53705 Madison , WI
Phone: 608-263-0313
US
Facilities: 
The mission of the UW Carbone Cancer Center (UWCCC) Flow Cytometry Laboratory (Flow Lab) is to provide cutting-edge instrumentation, technical expertise and flow cytometry education to the researchers of the UWCCC.

 

The Flow Lab was established in 1988 as a resource for the University of Wisconsin campus. 

 

The facility maintains two laboratories-- the main facility located in the Wisconsin Institutes for Medical Research (WIMR) and a satellite facility in the McArdle Laboratory. Like us on Facebook

 

Learn more about the Flow Lab's Scientific Accomplishments.
 
 
Policies
Contact Name: 
Dagna Sheerar, Manager
Phone number: 
608-263-0313

UWCCC Small Animal Imaging Facility

Location

1111 Highland Ave
53705 Madison , WI
Phone: 608-263-0959
Wisconsin
UWCCC Small Animal Imaging Facility
Location Information
Street Address: 
Wisconsin Institutes for Medical Research
1111 Highland Ave
53705 Madison , WI
Phone: 608-263-0959
US
Facilities: 

The mission of the UW Small Animal Imaging Facility (SAIF) is to provide innovative, state-of-the-art, affordable, noninvasive, high-resolution, in-vivo and ex-vivo imaging support to UW Carbone Cancer Center (UWCCC) members, UW researchers, and industries that use small animal models in their research.

 

Consultations
Facility director Jamey Weichert and manager Justin Jeffery provide consultations to investigators to determine which imaging methodologies are most suited to meet their research objectives.
 
The following are examples of parameters that can be tailored to answer your specific questions:
 
  • Animal diet and temperature
  • Anesthesia
  • Imaging modality
  • Contrast agent
  • Imaging time points
  • Scan duration
  • Gating
  • Temporal and spatial resolution
  • Static or dynamic framing
  • Image reconstruction

 

The director and manager can provide guidance to your overall study design, for example, whether to include control groups or to determine the number of subjects for statistical significance. Lastly, we can train you to accurately and efficiently analyze your data using our free-of-charge post-processing computers.

 

We provide three powerful PCs equipped with up to 64GB of RAM, dual 3.1 GHz processors, NVIDIA Quadro 4000 graphics cards, on a 64-bit Windows 7 OS to facilitate 2-D and 3-D image viewing, manipulation, and quantitative analysis of large files. The workstations are furnished with several imaging software packages including Siemens Inveon Research Workplace, Image J, GIMP, Amira, VivoQuant, and IVIS’s Living Image. We can easily convert imaging data to a universal DICOM format if you choose to perform analysis on your own workstation.

 

Micro-imaging suite:

We have enjoyed tremendous institutional support for our first class 2000 square foot facility that was specifically designed for small animal and molecular imaging in the WIMR (Wisconsin Institutes for Medical Research) 1 tower, a 9-story research building attached to the hospital, and adjacent to the School of Pharmacy and the Waisman Research Institute. This new facility houses the Siemens microCATII, Siemens Inveon Hybrid microPET/CT, Perkin Elmer IVIS, Fluoptics Fluobeam, and Agilent 4.7T MRI and associated hyperpolarization apparatus. We have a designated area for image analysis with our high end workstations. We boast our own animal holding room which has strictly regulated temperature, humidity, pressure, and light cycles, and which contains passively ventilated rodent housing racks for holding radioactive animals and those involved in long-term tumor monitoring studies. The new WIMR complex is strategically located adjacent to the new animal vivarium where non-radioactive animals involved in imaging studies are housed. This preclinical and molecular imaging suite is designed with translational research in mind as supported by our lab neighboring the clinical research GE Discovery VCT and GE Discovery 710 PET/CT scanners. Also next to the small animal imaging suite are the cyclotron, radiochemistry, and radiopharmacy facilities which provide expertise on PET agent synthesis in a collaborative and fee-for-service basis. The small animal imaging director and manager can coordinate radionuclide and radiotracer synthesis with the Cyclotron Group led by Drs. Jerry Nickles and Todd Barnhart in the Medical Physics Department. Alternatively, agents may be acquired from commercial sources such as PETNET or IBA Molecular.

 

Policies
Contact Name: 
Justin Jeffery, Manager
Phone number: 
608-263-0959

VA Hospital Confocal Facility

Location

2500 Overlook Terrace
53705 Madison , WI
Phone: (608) 256-1901
Wisconsin
William S. Middleton Memorial Veterans Hospital
Location Information
Street Address: 
William S. Middleton Memorial Veterans Hospital
2500 Overlook Terrace
53705 Madison , WI
Phone: (608) 256-1901
US
Facilities: 

The William S. Middleton Memorial Veterans Hospital is a highly affiliated acute care facility providing comprehensive tertiary care in medicine, surgery, neurology, and psychiatry. Approximately 1,400 professional, administrative, and support personnel provide the widest range of services to the more than 34,000 veterans treated annually, either as inpatients or outpatients.

The hospital is affiliated with the University of Wisconsin's Medical School and Hospital, boasting affiliations and sharing agreements which are extremely active. The two institutions share many resources, including nuclear medicine, angiography, autopsy, and organ transplantation programs.

Policies
Contact Name: 
Robert Conhaim

Vision Research Core

Location

470 N. Charter St.
53706 Madison , WI
Phone: 608-263-6805
Wisconsin
Vision Research Core
Location Information
Street Address: 
682 Service Memorial Institute
470 N. Charter St.
53706 Madison , WI
Phone: 608-263-6805
US
Facilities: 
The Lakkaraju lab uses the Andor Revolution XD microscopy system (with high speed spinning-disk confocal, TIRF, and Mosaic capabilities) to study intracellular organelle trafficking (endosomes, lysosomes and autophagosomes) and follow endo- and exocytic events at the cell membrane in real time.  This is a shared instrumentation facility supported by the Department of Ophthalmology & Visual Sciences, the School of Medicine and Public Health and by grants from the Research to Prevent Blindness Foundation and Reeves Foundation for Macular Degeneration Research.

Our objective is to discover the cellular basis of blinding diseases such as age-related macular degeneration (AMD), which affects over 30 million people worldwide currently. The long-term goal of our research is to use this knowledge to develop novel therapies that could help prevent devastating vision loss.  Our research also seeks to understand whether mechanisms that underlie age-related macular degeneration share features with other diseases of aging such as Alzheimer’s disease, Parkinson’s disease, atherosclerosis, etc.  A common thread in these seemingly disparate diseases is abnormal cholesterol metabolism and disrupted lysosome-autophagic function.  
 

Andor Revolution XD Confocal inverted microscope

  • Nikon Objectives:
    • 100x Apo TIRF (Oil, NA = 1.49, WD = 0.12 mm) with Temperature Correction collar (for 23°C and 37°C)
    • 60x Plan Apo VC (Oil, NA = 1.4, WD = 0.13 mm)
    • 40x Plan Fluor (Oil, NA = 1.3, WD = 0.2 mm)
    • 20x Plan Fluor (Dry, NA = 0.5, WD = 2.1 mm)
  • Andor Laser Combiner (ALC): Four solid state lasers at 405, 488, 561, and 640 nm with 10 millisecond switch time and active blanking control to minimize unnecessary illumination.
  • Confocal emission bandpass filters:
    • DAPI: 447/60 (417-477nm)
    • GFP: 525/30 (510-540nm)
    • RFP: 607/36 (589-625nm)
    • Far-red (CY5): 685/40 (665-705nm)
  • Okolab CO2 cage incubator: Environmental regulation of temperature, CO2, and humidity to achieve cell culture conditions.  Temperature is thermostat-regulated to ± 0.1°C from 3° above ambient temperature up to 50°C.

Supported by the Department of Ophthalmology and Visual Sciences, the University of Wisconsin School of Medicine & Public Health, the Carl and Mildred Reeves Foundation for Macular Degeneration Research, and generous funding provided by the Schuette/Kraemer Family gift.

 

Contact Name: 
Li Xuan Tan
Phone number: 
608-263-6805

Waisman Center CMN Core

Location

1500 Highland Avenue
53705 Madison , WI
Wisconsin
Waisman Center Cellular and Molecular Neuroscience Core
Location Information
Street Address: 
Waisman Center Cellular and Molecular Neuroscience Core
1500 Highland Avenue
53705 Madison , WI
US
Facilities: 

The Waisman Center is a comprehensive, multidisciplinary center at the University of Wisconsin-Madison devoted to the advancement of knowledge about intellectual and developmental disabilities (IDD) and neurodegenerative diseases. The Waisman Center is a fully integrated program that supports investigators conducting biological and behavioral research on IDD, trains students and junior scientists in fields relevant to IDD, trains clinicians and provides clinical services for persons with IDD, and conducts continuing education and technical assistance programs.

The objective of the Waisman Center's cellular and molecular neuroscience (CMN) core is to support the research objectives and scientific progress of investigators both in-house and across campus. The CMN core provides services and specialized equipment to perform cellular and molecular neuroscience research on human and animal tissues. We provide experimental design consultation and training on our equipment. The CMN core focuses on fluorescence quantitation and image analysis (Confocal and fluorescence/brightfield imaging, Stereology, Flow Cytometry, phosphorimaging, and real time PCR). In house we also support the production of induced Pluripotent Stem (iPS) cells from diseased patients. The CMN core provides genomic and proteomic analysis by enabling user access to other campus cores including the UW Biotechnology Center (UWBC) and the UW Comprehensive Cancer Center (UWCCC) in a subsidized partnership for IDDRC users. Thus the CMN core facilitates exploration of gene expression technology (NexGen, Gene Chip Analysis at UWBC), Mass spectrometry, genomic analysis and DNA synthesis/sequencing (UWBC), and small molecule screening (UWCCC).

Policies
Contact Name: 
Karla Knobel (Facility Manager)
Phone number: 
(608) 890-2280

Wisconsin Veterinary Diagnostic Laboratory

Location

445 Easterday Lane
53706 Madison , WI
Phone: 608-262-5432
Fax: 847-574-8085
Wisconsin
Wisconsin Veterinary Diagnostic Laboratory Electron Microscopy Facility
Location Information
Street Address: 
Wisconsin Veterinary Diagnostic Laboratory
445 Easterday Lane
53706 Madison , WI
Phone: 608-262-5432
Fax: 847-574-8085
US
Facilities: 

The Wisconsin Veterinary Diagnostic Laboratory, with facilities located in Madison and Barron, is a full service laboratory serving veterinarians from Wisconsin and throughout the United States.
WVDL is accredited by the American Association of Veterinary Laboratory Diagnosticians and provides a broad range of diagnostic services to the production, companion, and exotic animal industries. WVDL performs surveillance tests for a variety of animal diseases (e.g., brucellosis) and performs tests to qualify animals, semen, and embryos for interstate and international shipment.

Access to Facilities: Hours are 7:45am-4pm M-F  

 

Policies

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