The mission of the UW Small Animal Imaging Facility (SAIF) is to provide innovative, state-of-the-art, affordable, noninvasive, high-resolution, in-vivo and ex-vivo imaging support to UW Carbone Cancer Center (UWCCC) members, UW researchers, and industries that use small animal models in their research.
Facility director Jamey Weichert and manager Justin Jeffery provide consultations to investigators to determine which imaging methodologies are most suited to meet their research objectives.
The following are examples of parameters that can be tailored to answer your specific questions:
Animal diet and temperature
Imaging time points
Temporal and spatial resolution
Static or dynamic framing
The director and manager can provide guidance to your overall study design, for example, whether to include control groups or to determine the number of subjects for statistical significance. Lastly, we can train you to accurately and efficiently analyze your data using our free-of-charge post-processing computers.
We provide three powerful PCs equipped with up to 64GB of RAM, dual 3.1 GHz processors, NVIDIA Quadro 4000 graphics cards, on a 64-bit Windows 7 OS to facilitate 2-D and 3-D image viewing, manipulation, and quantitative analysis of large files. The workstations are furnished with several imaging software packages including Siemens Inveon Research Workplace, Image J, GIMP, Amira, VivoQuant, and IVIS’s Living Image. We can easily convert imaging data to a universal DICOM format if you choose to perform analysis on your own workstation.
We have enjoyed tremendous institutional support for our first class 2000 square foot facility that was specifically designed for small animal and molecular imaging in the WIMR (Wisconsin Institutes for Medical Research) 1 tower, a 9-story research building attached to the hospital, and adjacent to the School of Pharmacy and the Waisman Research Institute. This new facility houses the Siemens microCATII, Siemens Inveon Hybrid microPET/CT, Perkin Elmer IVIS, Fluoptics Fluobeam, and Agilent 4.7T MRI and associated hyperpolarization apparatus. We have a designated area for image analysis with our high end workstations. We boast our own animal holding room which has strictly regulated temperature, humidity, pressure, and light cycles, and which contains passively ventilated rodent housing racks for holding radioactive animals and those involved in long-term tumor monitoring studies. The new WIMR complex is strategically located adjacent to the new animal vivarium where non-radioactive animals involved in imaging studies are housed. This preclinical and molecular imaging suite is designed with translational research in mind as supported by our lab neighboring the clinical research GE Discovery VCT and GE Discovery 710 PET/CT scanners. Also next to the small animal imaging suite are the cyclotron, radiochemistry, and radiopharmacy facilities which provide expertise on PET agent synthesis in a collaborative and fee-for-service basis. The small animal imaging director and manager can coordinate radionuclide and radiotracer synthesis with the Cyclotron Group led by Drs. Jerry Nickles and Todd Barnhart in the Medical Physics Department. Alternatively, agents may be acquired from commercial sources such as PETNET or IBA Molecular.