Zeiss 510 Meta Confocal

Basic Information
Manufacturer: 
Zeiss
Name/Model #: 
510
Type: 
Confocal microscope
Location Information
Contact Information
Contact Name: 
Julie Last
Phone number: 
608-262-3613
Advanced Information
Features & Accessories: 

The Materials Science Center has a Zeiss 510 Meta confocal microscope with an Axiovert 200M inverted microscope.  This instrument is equipped with 2 PMT detectors and the META detector.  With the META detector, the emission is projected onto 32 channels for spectral separation. These detectors allow for the simultaneous imaging of several different fluorophores and with the META detector imaging is not limited by the available emission filters. The microscope has fluorescence capabilities with DAPI, FITC and Rhodamine filter cubes.  DIC imaging is possible with the 20x objective. Confocal images can be taken simultaneously with transmission images.  The microscope is also equipped with a motorized x – y stage. In addition, this system can be easily configured for reflectance confocal to image non-transparent samples.  

Instrument details:

Mercury Lamp:

DAPI, FITC and Rhodamine filters

 

Excitation Sources:

Argon: 458, 477, 488, 514

DPSS: 561

HeNe: 633

 

Excitation Dichroics: (KP = low pass)

NT 80/20 (passes 80% of the light)

488/561/633

KP 700/488

KP 700/561

458/514

458

488/561

KP 650

 

Objectives:

1)    A-Plan 10x/0.25air, WD = 4.5  

2)    C-Apochromat 10x/0.45 water, WD = 1.8

3)    Plan Apochromat 20x/0.8,WD = 0.55

4)    C-Apochromat 40x/1.2water, WD = 0.28

 

Emission filters: (KP = low pass, BP = band pass, LP = high pass)

Channel S: 32 channel META detector

Channel 2: KP 685, BP 390 – 465, BP 435 – 485, BP 480 – 520, BP 500 – 530, BP 500 – 550

Channel 3: LP 505, BP 510/20, BP 500 – 550, BP 535 – 590, BP 575 – 615, BP 650 – 710