The new IVIS Spectrum (Caliper Life Sciences) is capable of both bioluminescence and fluorescence scanning and is used for non-invasive longitudinal monitoring of cancer progression, metastatic cell trafficking, and gene expression patterns in living animals. An optimized set of high efficiency filters and spectral un-mixing algorithms affords noninvasive imaging of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using a digital mouse atlas. The Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal
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