Nikon N-SIM

Basic Information
Name/Model #: 
Super-resolution microscope
Location Information
Contact Information
Contact Name: 
Elle Kielar Grevstad, Director
Phone number: 
Advanced Information
Features & Accessories: 

Lasers: 405nm/488nm/561nm/640nm
Camera: Andor iXon 897 EMCCD
Microscope: Motorized inverted microscope ECLIPSE Ti-E, Perfect Focus System, Motorized XY stage with encoders, Piezeo Z stage, Tokai Hit, Environmental Chamber for Live Cell Imaging
Software: NIS-Elements Ar with N-Sim analysis












In structured illumination microscopy (SIM), the ultrastructure is derived from the moiré pattern produced when illuminating the specimen with a known high-frequency patterned illumination. Nikon’s Structured Illumination Microscope (N-SIM) realizes super resolution of up to 115 nm in multiple colors. In addition, it can continuously capture super-resolution images at a temporal resolution of 0.6 sec/frame, enabling the study of dynamic interactions in living cells.



Lateral resolution (FWHM of beads in xy)115 nm* in 3D-SIM mode
Axial resolution (FWHM of beads in z)269 nm* in 3D-SIM mode
Image acquisition timeUp to 0.6 sec/frame (TIRF-SIM/2D-SIM)
Up to 1 sec/frame (Slice 3D-SIM)
(needs more 1-2 sec. for calculation)
Imaging modeTIRF-SIM (TIRF XY super resolution)
2D-SIM (XY super resolution, up to 3μm deep)
Slice 3D-SIM (XYZ super resolution, up to 20 μm deep)
Stack 3D-SIM (XYZ super resolution, up to 50 μm deep)
Multi-color imagingUp to 5 colors
Compatible LaserStandard: 488 nm, 561 nm
Option: 405 nm, 458 nm, 514 nm, 532 nm, 640 nm
Laser combination: 
405 nm/488 nm/514 nm/532 nm/561 nm, 
405 nm/488 nm/514 nm/561 nm/640 nm,
458 nm/488 nm/514 nm/532 nm/561 nm, 
458 nm/488 nm/514 nm/561 nm/640 nm
Compatible microscopeMotorized inverted microscope ECLIPSE Ti-E
  • Perfect Focus System
  • Motorized XY stage with encoders
  • Piezo Z stage
Compatible objectiveCFI SR Apochromat TIRF 100×oil (NA1.49)
CFI Apochromat TIRF 100×oil (NA1.49)
CFI SR Plan Apochromat IR 60×WI (NA1.27)
CFI Plan Apochromat IR 60×WI (NA1.27)
CameraAndor Technology iXon3 897 EMCCD camera
SoftwareNIS-Elements Ar/
NIS-Elements C (for Confocal Microscope A1+/A1R+)
Both require optional module software NIS-A N-SIM Analysis
Operating conditions20 ºC to 28 ºC ( ± 0.5 ºC)