Flexible bandwidth multispectral microscopy. The Nuance® FX multiplex biomarker imaging system is specifically tailored to meet the majority of all brightfield and fluorescence microscopy imaging applications. The system utilizes an optimized, high-throughput tunable filter distinctly matched to the bandwidths of common molecular markers. This solid-state filter is electronically switchable to maximize either sensitivity or throughput, enabling the Nuance FX to provide either chromogenic or fluorescence-based imaging. Nuance acquisition software incorporates easy to use tools for building custom spectral libraries, as well as powerful unmixing and analysis algorithms allowing spectral characterization and quantitation for each of the multi-labeled components in an image. inForm advanced image analysis software is also an available option for rapid analysis of targeted tissues and batch processing of user-specified regions across multiple images.
Company Link to Description
Brightfield (BF) Fluorescence(Fl)
Single slide capacity
Up to 14 channels
Nuance and inForm
Ordinal & Continuous
Biomarker quantification * Co-localization & quantification
* Per-tissue analysis (epith. vs. stroma) *
Subcellular quantification *
Per-cell data *
Tissue structure (vessel, glomerulus, etc) counting
Nuance User's Manual
The UW Microscopy Portal is run by the Laboratory for Optical and Computational Instrumentation of the University of Wisconsin-Madison. © 2017 Board of Regents of the University of Wisconsin System. Feedback, questions or accessibility issues: contact us.